In the last decade, a novel oncogene, chromodomain helicase/ATPase DNA binding protein 1-like (CHD1L) gene, has been isolated from the 1q21 amplicon. Amplification of 1q21 is a frequent genetic alteration in a number of solid tumors (5). The use of EBV-related detection alone for NPC screening cannot meet the requirements as a diagnostic marker therefore, finding the molecular biomarkers for NPC is necessary to improve the treatment regimes and predict the prognosis for patients with NPC. The present clinical screening method for NPC is mainly dependent on detecting Epstein-Barr virus (EBV) infection in the serum, but not all NPC carcinogenesis is associated with EBV furthermore, EBV infection can be observed in a number of other diseases (4). NPC, often accompanied by early lymph node metastasis, is hard to detect due to the hidden location and lack of clinical manifestations at the early stages (3). Worldwide, NPC is most prevalent in China, with an age-standardized incidence rate of >20 per 100,000 individuals (2). Kaplan‑Meier survival analysis demonstrated that patients with CHD1L‑positive NPC had significantly shorter overall survival (P Introduction Nasopharyngeal carcinoma (NPC) is derived from the epithelial cells that cover the surface of and line the nasopharynx (1). In addition, positive CHD1L expression was strongly associated with an advanced clinical stage (P=0.016), recurrence (P=0.002) and the metastasis status (P=0.031) of NPC.
Immunohistochemical analysis showed that 88 of 133 (66.2%) paraffin‑embedded NPC biopsies exhibited positive expression of CHD1L, but all non‑cancerous nasopharyngeal specimens were negative for CHD1L expression. The correlations of CHD1L expression status with clinicopathological features and prognosis were investigated. CHD1L protein expression was examined by performing western blot analysis of 30 fresh NPC tissues and conducting immunohistochemistry tests of 133 NPC samples between Decemand December 1, 2009. The aim of this study was to evaluate the clinical significance of CHD1L positivity in NPC. Recent studies have implicated the role of chromodomain helicase/ATPase DNA binding protein 1‑like (CHD1L) gene as a novel oncogene however, the functional role of CHD1L in NPC remains unknown. Abstract: Nasopharyngeal carcinoma (NPC) is a malignancy with a high metastatic ability.
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